The Bruggeman lab

Our new paper on single-molecule RNA FISH in mammalian cells is published

You can find it here: paper. In this study, we use confocal microscopy to simultaneously measure the volume and number of transcripts, per nucleus and cytoplasm, of single cells. This allowed us to show that the transcription concentration remains fixed across cells of different sizes – likely at different extends of cell cycle progression. As consequence of this is that the noise in transcript numbers is much greater than when transcript concentrations are considered. We could fully account for all the statistical data, using variance decomposition.